I learned that Fredrick Sanger won two noble prizes, noble in chemistry 1958 for the structure of insulin and noble in chemistry 1975 for DNA sequencing. He also invented DNA sequencing. She told us that large scale DNA sequencing uses the dideoxy method with some improvements for increased efficiency and speed. Before we left to go to the lab we sequenced DNA off of DNA sequence paper from a while back. It was fun because we were all confused at the beginning but then got used to it. But it didn't work because Dr. Fineschi gave us the wrong sequence. But I learned that scientists sequence 200 genes at a time because there are a lot of genes to sequence.
When we went to the lab and extracted DNA from our mutant in the environmental plates and used PCR to amplify the rpoB gene. Next we mixed the 3 different colonies in 3 different tubes with lysis in it. After that we put the sample tubes into the thermocycler because the lysis solution needs to be heated up in order to be activated. As we waited, we put 40 mL of water into 3 separate tubes labeled for red, blue, and environmental. After we put the water in, we put the diluted samples into the tube that matched the label. Then we used a pipette and inserted 2.5 mL of the lysed dilution into the tac bead tube. Next, we added 17.5 mL of primer and then 5.0 mL of water. Next, we put it into the centrifuge for the short cycle and then put it in the thermocycler.
|Dawn, the TA, Helping my Partner Sofi|
At dinner we all ate in the main dining hall. I ate fruit, rice, chicken nuggets, and for desert I had one scoop of cotton candy ice cream in a cone. At dinner we made a lot of jokes and laughed almost the whole time we were there. I ate with Alexa, Jimmy, Oyin, Dani, and Kaitlyn. Then we left and I went to my room for a while until I went to go play soccer with Jimmy and some more people from the program. I had fun and we stayed until 8:45 PM. Sadly my team lost but it's okay because I still had fun playing with them. But ending the night with my Chicago Family is the best thing every night.