Thursday, July 17, 2014

Mutation Frequency, Gym, and Dinner

The Group Picture
Normal time I wake up is 7:45 so I can get ready to get breakfast and be on my way slowly to class because I get to my building early so I just take my time when I walk. Instead of going to the classroom first we went to the lab. In the lab we counted the bacterial colonies on the Rifampicin plate we were waiting for to be finished. We calculated the bacterial concentration so we can find the mutation frequency using the cfu/mL of the LB plate that was already done and divided the number of bacterial colonies of the Rifampicin plate. Then we lysed one of the mutant colonies from the Rifampicin  plates, put it in the lysis tube, and used a centrafuge. After using the centrafuge we put it in the thermocycler for 15 minutes. From the thermocycler, we lysed the bacteria, and put it back into the centrafuge. We were taking the DNA from the bacteria in this process. Then before lunch we went into the classroom for a lecture about human pathogens, pathogenic bacteria, why they make us sick, and more about bacterial toxins. I learned that there was a hamburger outbreak of gastrointestinal disease in 1982.

At lunch I had salad, fruit, pita bread with humus, and mac-n-cheese. I sat with Alexa, Katherine, Eugenia, and Bella. After we were all finished eating most of us departed from each other. Alexa and Katherine walked halfway back to class with me since their class was on the way to my class. When I got to my class we started in the lab again. This time we took our samples out of the centrafuge and we took the DNA out and out it into another tube. Then we added loading dye to the DNA in order to load it in the gel with a pipette. My group sample didn't work so we used someone else's sample. After class I stayed for 20 minutes and I went back to my dorm and laid down for a bit before I went to the gym with Alexa. My roommate was supposed to come with us but she couldn't come because she wasn't feeling good. As we got to the gym I went to play basketball with some people that were there already and Alexa ran on the treadmill . Their names are Derrick, Eric, and Cam. We played on teams and my team won the first game and then we played another game with more people but I had to be subbed out because I had to get ready for dinner with people from UChicago.

Crème Brulee
At the dinner we met Troy an admissions officer, Peter another admissions officer, Teddy, Eric, Leilani, and Lily. The restaurant we went to is The Capital Grille. Before everyone started talking to each other we introduced ourselves. I sat by Lily and Peter but I mostly talked to Lily for the majority of the time. We both had interesting stories about our siblings. She also shared her interest in music and TV shows.  We also talked about the course I'm taking and what I do in this course. Then we ordered and I got the 

Sirloin w/ Lobster Tails
sirloin with lobster tails and for desert I had Creme Brulee. Out of the three Creme Brulees at three different restaurants and the Creme Brulee from The Capital Grille is bar far the best one. After the dinner we took a group photo like usual and then took cabs back to campus and luckily we got back before curfew.

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